a,
Pie charts showing how many of our 547 complexes have the
indicated percentages of their subunits appearing in individual MIPS
complexes or complexes identified by other affinity-based purification
studies13,14. b,
Precision and homogeneity (see text) in comparison to MIPS complexes
for three large-scale studies. c,
The relationship between complex size (number of different subunits)
and frequency. d,
Graphical representation of the complexes. This Cytoscape/GenePro
screenshot displays patterns of evolutionary conservation of complex
subunits. Each pie chart represents an individual complex, its relative
size indicating the number of proteins in the complex. The thicknesses
of the 429 edges connecting complexes are proportional to the number of
protein–protein interactions between connected nodes. Complexes lacking
connections shown at the bottom of this figure have <2 interactions
with any other complex. Sector colours (see panel f)
indicate
the proportion of subunits sharing significant sequence similarity to
various taxonomic groups (see Methods). Insets provide views of two
selected complexes—the kinetochore machinery and a previously
uncharacterized, highly conserved fructose-1,6-bisphosphatase-degrading
complex (see text for details)—detailing specific interactions between
proteins identified within the complex (purple borders) and with other
proteins that interact with at least one member of the complex (blue
borders). Colours indicate taxonomic similarity. e,
Relationship between protein frequency in the core data set and degree
of connectivity or betweenness as a function of conservation. Colours
of the bars indicate the evolutionary grouping. f,
Colour key
indicating the taxonomic groupings (and their phylogenetic
relationships). Numbers indicate the total number of ORFs sharing
significant sequence similarity with a gene in at least one organism
associated with that group and, importantly, not possessing similarity
to any gene from more distantly related organisms.
[from Krogan et al, Nature,
2006, Mar 30,440(7084):637-43]