a, Pie charts showing how many of our 547 complexes have the indicated percentages of their subunits appearing in individual MIPS complexes or complexes identified by other affinity-based purification studies13,14. b, Precision and homogeneity (see text) in comparison to MIPS complexes for three large-scale studies. c, The relationship between complex size (number of different subunits) and frequency. d, Graphical representation of the complexes. This Cytoscape/GenePro screenshot displays patterns of evolutionary conservation of complex subunits. Each pie chart represents an individual complex, its relative size indicating the number of proteins in the complex. The thicknesses of the 429 edges connecting complexes are proportional to the number of protein–protein interactions between connected nodes. Complexes lacking connections shown at the bottom of this figure have <2 interactions with any other complex. Sector colours (see panel f) indicate the proportion of subunits sharing significant sequence similarity to various taxonomic groups (see Methods). Insets provide views of two selected complexes—the kinetochore machinery and a previously uncharacterized, highly conserved fructose-1,6-bisphosphatase-degrading complex (see text for details)—detailing specific interactions between proteins identified within the complex (purple borders) and with other proteins that interact with at least one member of the complex (blue borders). Colours indicate taxonomic similarity. e, Relationship between protein frequency in the core data set and degree of connectivity or betweenness as a function of conservation. Colours of the bars indicate the evolutionary grouping. f, Colour key indicating the taxonomic groupings (and their phylogenetic relationships). Numbers indicate the total number of ORFs sharing significant sequence similarity with a gene in at least one organism associated with that group and, importantly, not possessing similarity to any gene from more distantly related organisms. [from Krogan et al,  Nature, 2006, Mar 30,440(7084):637-43]