Schematic representation of approach. The large squares labeled B1, B2, etc. represent the geographic sites (buckets). Each bucket has sequence and environmental feature data associated with it. (A) Mapping quantitative environmental features [salinity (ppt), sample depth (position in water column from which the sample was collected), water column depth (measured from surface to floor), and chlorophyll]. (B) Metagenome-derived metabolism at different levels of resolution (see Materials and Methods). Reads are color-coded according to their corresponding pathway elements (shapes). Different pathways are represented by different shapes (square, circle, etc.). All of the instances of a particular pathway are summed and normalized to compute the pathway score. (C) Schematic representation of DPM (see details in text). (D) Schematic representation of CCA (see details in text).
[from T. A. Gianoulis, J. Raes, P. V. Patel, R. Bjornson, J. O. Korbel, I. Letunic, T. Yamada, A. Paccanaro, L. J. Jensen, M. Snyder, P. Bork, and M. B. Gerstein
Quantifying environmental adaptation of metabolic pathways in metagenomics, Proceedings of the National Academy of Sciences, vol. 106, iss. 5, pp. 1374-1379, 2009